This Axio Scope A1 System manufactured by Zeiss is configured for fluorescence applications. This system was used over the course for about a year for customer demonstrations. The optics are fully functional, and the system has been cleaned.
- Objective Lenses: Infinity corrected N-Achroplan 5x, 10x, 20x, 50x oil, 100x oil objectives.
- N-Achroplan 5x/0.15, WD = 12mm
- N-Achroplan 10x/0.25, WD = 6.5mm
- N-Achroplan 20x/0.45, WD = 0.63mm
- N-Achroplan 50x/1.0 Oil, WD = 0.22
- N-Achroplan 100x/1.25 Oil, WD = 0.29mm
- Viewing Head: Ergonomic head allows the eyepieces to be adjusted between 5 and 30º of inclination. FN = 23. Upright image with prism switch and 100:0/ 0:100 light to camera/ eyepieces. Camera port has interface 60N.
- Eyepieces: Widefield 10x / 23 focusing eyepieces, accepts 26mm diameter reticle. Diopter adjustment +/-5. Interpupillary distance adjustable from 48-75mm.
- Total Magnification: 50x, 100x, 200x, 500x, 1000x.
- Specimen Stage: 220mm W x 170mm D right hand mechanical stage with X-Y travel of 75mm x 50mm, hard-coated anodized surface with rackless X-guide positioned at bottom, hidden gear in the Y-guide. Right drive 135mm (extendable by 15mm), with friction setting. Includes specimen holder for one-hand operation with left spring lever.
- Focusing: Coarse and fine focusing. Z focus range is 24mm.
- Condenser: Achromatic, aplanatic condenser 0.9 with front lens switchable on the left and right and a 5 position modulator disc. Condenser has 4 centerable posisionts with Ph1, Ph2, Ph3, and dark-field stop 0.75. One bright field position with aperture stop. WD = 1.0
- Illumination: Transmitted light illumination white LED. 100~240V.
- Stand Features: Stand is configured for transmitted light and fluorescence with an encoded 6-position nosepiece.
- Fluorescence: The illumination system has 3 solid state LED lamps:
- Green (565nm) for excitation of Cy3, TRITC, DsRed and similar dyes
- Blue (470nm) for excitation of eGFP, Fluo4, FITC and similar dyes
- UV (385nm) for excitation of DAPI, Alexa 405, Hoechst 3325
- Fluorescence filters: 3 filter sets are included:
- UV: compatible with a broad range of LEDs with peaks between 355-405nm, intended for fluorophores such as DAPI.
- Blue: intended for fluorophores such as FITC and eGFP
- Green intended for fluorophores such as mCherry and Texas Red
- Warranty: Can be returned for 60 days.
- Size & Weight: 9.5" W x 19.1" H (without camera) x 17" D, 20 lb.
